同型对照抗体

内参抗体



  1. 敏感性比较


一抗:
        A. 0.1 μg/ml THETM β-Actin mAb(Mouse)(金斯瑞, A00702)
        B. 0.1 μg/ml β-Actin mAb (Mouse) (X公司)
二抗:
         Goat Anti-Mouse IgG (H&L) [HRP] pAb (金斯瑞, 1:10,000, A00160)
        信号检测:LumisensorTM HRP底物试剂盒(金斯瑞, L00221V500)

  2. 免疫荧光分析


一抗:
        0.1 μg/ml THETM β-Tubulin mAb (金斯瑞, A01410)
二抗:
        荧光偶联亲和纯化 anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)

  3. 荧光Western Blotting


一抗:
        0.1 μg/ml GAPDH Antibody, pAb, Goat (金斯瑞, A00191)
泳道 1-8:
         5.0, 2.5,1.25, 0.62, 0.31, 0.16, 0.08及0.04 μg Hela cell裂解液
        使用试剂盒:One-Step WesternTM Fluorescent Kit (金斯瑞, L00397)

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